Research analist

We are looking for an enthusiastic and motivated technician to contribute to the development of new immune therapies for cancer patients. As a research technician, you work alongside PhD students, postdocs and other research technicians to improve immunotherapy against cancer. In this translational project, you focus on enhancing allogeneic Natural Killer (NK) cell therapy for ovarian cancer, and contribute to translating this research into the clinic.

Within this project, you are responsible for collecting umbilical cord blood from the operating room and isolating CD34+ stem cells from this blood. Through a culturing process, you will expand and differentiate these CD34+ stem cells into large quantities of NK cells. These NK cells are used for functional in vitro and in vivo experiments. Additionally, you will work on improving the specificity, activity, and expansion of these NK cells through viral transduction of chimeric antigen receptors (CAR). These CARs are specifically targeted against proteins that are highly expressed on ovarian cancer cells, allowing these CAR‑NK cells to operate more precisely.

Tasks and responsibililties

Collecting and processing umbilical cord blood from the operating room and delivery rooms.

Sterile isolation and culturing of primary cells and cell lines.

PCR analysis and cloning CAR constructs.

Producing plasmids and (lenti and retro) viruses.

Performing transfections and transductions.

Conducting flow cytometry and functional assays.

Independently preparing protocols, conducting experiments and analyzing data.

Reporting results in meetings and through presentations to the department.

Ensuring accurate documentationview.

Together, your work will contribute to the further development of CAR‑NK cell therapy, reflected in scientific publications and the translation to clinical trials.

Do you want to contribute to the development of new cancer therapies? Come and join our team!

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