Job Description
Job Description: Cell Processing — PRP & Bone Marrow–Derived Cell Fractions
Organisation:
ALIV Regenerative Wellness
Location:
Pune |
Type:
Full-time |
Mode:
On-site
Reports to:
Lab/Clinical Operations Lead
1 Month TRAINING in PUNE
About ALIV
ALIV is a compliant regenerative wellness clinic focused on
Autologous Cell Therapy ,
PRP/PRFM , and
targeted IV therapies , delivered with strict ethics and SOPs.
Role Summary
Hands-on technician to
process PRP/PRFM
and
bone marrow–derived autologous cell fractions (minimally manipulated)
with impeccable aseptic technique, documentation, and turnaround time. You’ll partner with clinicians to ensure safe, consistent, and reproducible outputs for patient procedures.
Key Responsibilities
Sample Handling & Chain of Custody
Receive, label, and track blood and bone-marrow aspirate; maintain time/temperature controls and transport logs.
Verify consent, requisitions, and kits; ensure traceability from collection to reinfusion.
PRP / PRFM Processing
Perform anticoagulation, centrifugation per protocol (soft/hard spins), plasma separation, platelet resuspension, PRFM activation.
Record volumes, spin parameters, platelet yield/concentration, and lot numbers of disposables.
Bone Marrow–Derived Cell Fraction Processing
Process aspirate using validated
closed/open systems
(e.g., RBC depletion, density separation).
Isolate mononuclear/cell fractions as per SOP; prepare final product for clinical use.
Asepsis, QC & Release
Work under
Class II biosafety cabinet/laminar flow ; follow sterile technique and PPE.
Perform in-process QC:
TNC/platelet counts, viability (e.g., Trypan Blue), recovery %, hemolysis check , appearance.
Prepare batch records and
Certificate of Processing/Release
for clinician sign-off.
Equipment & Facility
Operate and maintain
centrifuges (fixed/swing), pipettes, cell counters/hemocytometer, vortex, incubators (if applicable) .
Daily/weekly
calibration, IQ/OQ/PQ logs , maintenance tickets, and environmental hygiene records.
Compliance & Safety
Adhere to
DCGI/ICMR guidance , clinic SOPs, biosafety, biomedical waste rules, and incident/AER reporting.
Participate in audits, CAPA, and periodic validation runs.
Inventory & Coordination
Manage stock of
kits, anticoagulants, reagents, disposables ; raise purchase requests proactively.
Coordinate OR/clinic schedules for timely processing and delivery.
Qualifications
B.Sc./M.Sc.
in Biotechnology/Microbiology/Biomedical/Allied Health
or DMLT/BMLT .
2–5 years
in a cell/hematology/transfusion, IVF, orthobiologics, or day-care lab (PRP/BM processing preferred).
Strong grasp of aseptic technique, centrifugation theory, and basic hematology counts.
Comfortable with
EMR/CRM/LIS , Google Workspace, and documentation discipline.
Languages:
English + Hindi/Marathi (or local) .
Skills & Traits
Meticulous, steady hands, and
SOP-first
mindset.
Data-accurate; converts protocols into repeatable outputs.
Calm under time pressure; clear communication with clinicians and nursing.
Integrity, patient privacy, and teamwork.
KPIs
Yield & viability
within spec;
turnaround time
adherence.
Documentation accuracy
(batch records, logs) and SOP compliance.
Zero contamination/incident rate ; successful audits.
Stockouts avoided; equipment uptime.
90-Day Outcomes
30 days:
Trained and certified on ALIV SOPs; independent PRP runs with complete records.
60 days:
Independent bone-marrow fractionation; QC dashboards live.
90 days:
≥95% runs within yield/viability specs; audit-ready documentation; proposes at least
2
process improvements.